Clathrin Heavy Chain (CLTC) (Heavy Chain) antibody

Catalog No. ABIN111496

This Mouse Monoclonal antibody specifically detects in WB and IHC (fro). It exhibits reactivity toward Human, Rat, Cow, Pig and Amphibian.

Quick Overview for Clathrin Heavy Chain (CLTC) (Heavy Chain) antibody (ABIN111496)

Target: Clathrin Heavy Chain (CLTC)

Reactivity: Human, Rat, Cow, Pig, Amphibian

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CHC5-9

Product Details

Binding Specificity: Heavy Chain

Specificity: Clone CHC5.9 represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. Refs. 2 and 3). Polypeptide Reacting: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues. Structures and Tissues Specifically Detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries). Reactivities on Cultured Cell Lines (tested so far): HeLa, SV-40, RVF-SMC.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Pig, Rat and Amphibia.

Purification: Gel filtration

Immunogen: Coated vesicles (Clathrin) of Bovine brain.

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry on Frozen Tissue: 1/10. (When reconstituted, dilute further with PBS, pH 7.4). Incubation Time: 1 h at RT. Immunoblotting (Western): 1/100-1/500 (ECL). Successful use in Immunoprecipitation was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details

Target: Clathrin Heavy Chain (CLTC)

Alternative Name: Clathrin Heavy Chain

Background: Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.Synonyms: CLH17, CLTC, CLTCL2, Clathrin heavy chain on chromosome 17, KIAA0034, Membrane Vesicle Marker

Gene ID: 281080

NCBI Accession: NP_776448

UniProt: P49951