CD163 antibody (FITC)

Catalog No. ABIN111836

The Mouse Monoclonal anti-CD163 antibody is suitable to detect CD163 in samples from Rat. It has been validated for FACS.

Quick Overview for CD163 antibody (FITC) (ABIN111836)

Target: CD163

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: ED2

Product Details anti-CD163 Antibody (FITC)

Specificity: This antibody recognises the CD163 cell surface glycoprotein, a 175 kDa molecule also known as ED2.

Purification: Affinity chromatography on Protein A

Immunogen: Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry (neat - 1/10): use 10 μL of the suggested working dilution to label 10^6 cellsin 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.2, containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD163

Target: CD163

Alternative Name: CD163

Background: CD163 is expressed by approximately 50 % of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

Gene ID: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7