LY75/DEC-205 antibody (Biotin)

Catalog No. ABIN111869

The Rat Monoclonal anti-LY75/DEC-205 antibody has been validated for FACS and IHC (fro). It is suitable to detect LY75/DEC-205 in samples from Mouse.

Quick Overview for LY75/DEC-205 antibody (Biotin) (ABIN111869)

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LY75/DEC-205 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: NLDC145

Product Details anti-LY75/DEC-205 Antibody (Biotin)

Specificity: Reacts with nonlymphoid dendritic cells: interdigitating cells (IDC), veiled cells and Langerhans cells, thymic epithelial cells. Monoclonal antibody NLDC-145 identifies Ia positive interdigitating cells, veiled cells and Langerhans cells of the skin and their in vitro counterparts. The antigen is expressed at high levels by dendritic cells and thymic epithelial cells. The antigen detected by NLDC-145 is an integral membrane glycoprotein with an apparent mass of 205 kDa, also known as DEC-205.

Purification: Affinity Chormatography.

Immunogen: Mouse lymph node tissue.

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on Acetone Fixed Frozen Sections: 3 μg/mL (1/300). Recommended Positive Control: Mouse Spleen. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.3 mg/mL

Buffer: PBS, pH 7.2 containing 5 mg/mL BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and following reconstitution at 2-8 °C. DO NOT FREEZE! This antibody is photosensitive and should be protected from light.

Target Details for LY75/DEC-205

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Alternative Name: CD205 / DEC-205

Background: Mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. DEC-205 is apparently a receptor involved in antigen-processing by dendritic cells.Synonyms: C-type lectin domain family 13 member B, CLEC13B, DEC205, LY75, Lymphocyte antigen 75, gp200-MR6

Gene ID: 17076

NCBI Accession: NP_038853

UniProt: Q60767