S100A8/A9 Complex (Calprotectin) antibody

Catalog No. ABIN111882

This anti-S100A8/A9 Complex (Calprotectin) antibody is a Mouse Monoclonal antibody detecting S100A8/A9 Complex (Calprotectin) in IHC (p), EIA and IHC (fro). Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for S100A8/A9 Complex (Calprotectin) antibody (ABIN111882)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8/A9 Complex (Calprotectin) antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2-70E-011

Product Details anti-S100A8/A9 Complex (Calprotectin) Antibody

Specificity: This antibody is ideally suited for the detection of early inflammatory macrophages and thus for the classification of acute stage inflammation in tissue sections and in smears, the characterization of tumorous tissues and the in vitro monitoring of peripheral blood cell cultures. Clone 27E10 is unique in that it recognizes an epitope on the MRP8/14 heterocomplex that is not exposed on the individual subunits MRP8 or MRP14. The antibody reacts with Human subpopulations of macrophages, monocytes and granulocytes, peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Antigen Distribution Isolated Cells: Monocytes carry the antigen both on the surface and intracellularly, granulocytes exhibit it only intracellularly. Up to 80 % of monocytes in early cultures (24-48h) are positive. No reaction has been seen with lymphocytes or platelets. Tissue Sections: The antigen is found in macrophages in the red pulp of the spleen and liver. It is strongly expressed in macrophages from acute inflamed tissues (peridontitis, contact eczema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen. It is normally absent on resident mononuclear phagocytes in healthy tissues (skin, gut, thymus).Antigen Distribution

Cross-Reactivity (Details): Species reactivity (tested):Human. The antibody also stains a subpopulation of macrophages of Rhesus Monkey and Bovine tissues. It does not react with Swine tissues.

Purification: Affinity Chromatography on Protein A

Immunogen: Cultured Human monocytes. Remarks: The antigen is MRP8/14 (Calprotectin), the epitope involves parts of both subunits MRP8 and MRP14.

Isotype: IgG1

Application Details

Application Notes: ELISAImmunohistochemistry on Frozen Sections: 0.25 μg/mL (1/800)Immunohistochemistry on Paraffin Sections: 1 μg/mL (1/200), Proteinase K pre-treatmentfor antigen retrieval is recommended. Suggested Positive Control: Human tonsil. Has been described to work in FACS and Dot blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer’s hemalum

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water (= 0.2 mg/mL Stock Solution).

Concentration: 0.2 mg/mL

Buffer: Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!.

References for anti-S100A8/A9 Complex (Calprotectin) antibody (ABIN111882)

Ghavami, Rashedi, Dattilo, Eshraghi, Chazin, Hashemi, Wesselborg, Kerkhoff, Los: "S100A8/A9 at low concentration promotes tumor cell growth via RAGE ligation and MAP kinase-dependent pathway." in: Journal of leukocyte biology, Vol. 83, Issue 6, pp. 1484-92, (2008) (PubMed).

Altwegg, Neidhart, Hersberger, Müller, Eberli, Corti, Roffi, Sütsch, Gay, von Eckardstein, Wischnewsky, Lüscher, Maier: "Myeloid-related protein 8/14 complex is released by monocytes and granulocytes at the site of coronary occlusion: a novel, early, and sensitive marker of acute coronary syndromes." in: European heart journal, Vol. 28, Issue 8, pp. 941-8, (2007) (PubMed).

Target Details for S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Alternative Name: MRP8 / MRP14

Background: MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. The antigen is produced by the heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S 100 protein family.Synonyms: CAGA, CAGB, CFAG, Calgranulin A/B, Calprotectin, L1 Protein, MRP-14, MRP-8, P14, P8

Pathways: S100 Proteins