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Calprotectin antibody (Biotin)

S100A8/A9 Reactivity: Human WB, FACS, HPLC Host: Mouse Monoclonal 27E10 Biotin
Catalog No. ABIN2192017
  • Target See all Calprotectin (S100A8/A9) Antibodies
    Calprotectin (S100A8/A9)
    Reactivity
    • 30
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 24
    • 7
    Mouse
    Clonality
    • 24
    • 7
    Monoclonal
    Conjugate
    • 17
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    This Calprotectin antibody is conjugated to Biotin
    Application
    • 17
    • 11
    • 11
    • 10
    • 6
    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), High Pressure Liquid Chromatography (HPLC)
    Cross-Reactivity (Details)
    Cross reactivity: Mouse : No, Rhesus Monkey : Yes (subpopulation of macrophages)
    Sterility
    0.2 μm filtered
    Clone
    27E10
    Isotype
    IgG1
    Top Product
    Discover our top product S100A8/A9 Primary Antibody
  • Application Notes
    For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Human granulocytes control Negative Platelets, lymphocytes, HL-60 cells control
    Restrictions
    For Research Use only
  • Buffer
    PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Product should be stored at 4 °C Under recommended storage conditions, product is stable for one year.
    Expiry Date
    12 months
  • Kuhn, Sonntag, Sunderkötter, Lehmann, Vestweber, Ruzicka: "Upregulation of epidermal surface molecule expression in primary and ultraviolet-induced lesions of lupus erythematosus tumidus." in: The British journal of dermatology, Vol. 146, Issue 5, pp. 801-9, (2002) (PubMed).

    Hessian, Fisher: "The heterodimeric complex of MRP-8 (S100A8) and MRP-14 (S100A9). Antibody recognition, epitope definition and the implications for structure." in: European journal of biochemistry / FEBS, Vol. 268, Issue 2, pp. 353-63, (2001) (PubMed).

    Zwadlo, Schlegel, Sorg: "A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 2, pp. 512-8, (1986) (PubMed).

  • Target
    Calprotectin (S100A8/A9)
    Alternative Name
    Calprotectin (S100A8/A9 Products)
    Synonyms
    60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, Mrp14 antibody, MRP-14 antibody, p14 antibody, 60B8Ag antibody, AW546964 antibody, BEE22 antibody, Cagb antibody, GAGB antibody, L1Ag antibody, S100 calcium binding protein A9 antibody, S100 calcium binding protein A9 (calgranulin B) antibody, S100A9 antibody, S100a9 antibody
    Background
    The monoclonal antibody 27E10 recognizes an epitope specific for the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8, calgranulin-A) or S100A9 (MRP14, calgranulin- B). The calcium-binding migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60 % of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody 27E10 does not react with lymphocytes or platelets. Aliases S100A8/A9, MRP-8/MRP-14, calprotectin, calgranulin-A/calgranulin-B, L1-protein Immunogen Human blood monocytes
    Pathways
    S100 Proteins
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