S100A8 antibody (C-Term) (Biotin)

Catalog No. ABIN111893

The Mouse Monoclonal anti-S100A8 antibody is suitable to detect S100A8 in samples from Human, Rat and Pig. It has been validated for WB, FACS, IHC (p), EIA and IHC (fro).

Quick Overview for S100A8 antibody (C-Term) (Biotin) (ABIN111893)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: S13-67

Product Details anti-S100A8 Antibody (Biotin)

Binding Specificity: C-Term

Specificity: S 13.67 identifies the Ca2+-binding light subunit of the inflammatory L-1 protein complex, also called S100A8 or Calgranulin A. S 13.67 is useful for the identification of the 8 kDa S100A8 subunit in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. S13.67 detects a very distinct, as yet not characterized epitope of MRP8 (different from the 8-5C2 epitope). Antigen Distribution Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukaemia stains positively. Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP-8 increasingly during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8 / MRP14(TNF-alpha/IL-1 alpha induced inflammation of the skin).

Cross-Reactivity (Details): Species reactivity (tested):Human: MRP8 in stimulated monocytes and macrophages inlate phase or chronic inflammation.Rat, Cow, Pig (TNF-alpha/IL-1 alpha induced inflammation of the skin).

Purification: Affinity Chromatography

Immunogen: Cultured Human Monocytes. Remarks: The antigen is MRP8, the epitope is suspected in the N- or C-terminal portion of the peptide.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry. Immunohistochemistry on: Frozen sections: 1 μg/mL (1: 200)Paraffin sections: 5-10 μg/mL (1: 20-1: 50), no pretreatment for antigen retrieval necessary.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 0.5 mL distilled water to make stock solution.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8 / Calgranulin-A / MRP8

Background: MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A

Gene ID: 9606

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins