EPO antibody

Catalog No. ABIN111894

This anti-EPO antibody is a Mouse Monoclonal antibody detecting EPO in WB. Suitable for Human and Rat.

Quick Overview for EPO antibody (ABIN111894)

Target: EPO (Erythropoietin (EPO))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPO antibody is un-conjugated

Application: Western Blotting (WB)

Clone: AE7A5

Product Details anti-EPO Antibody

Specificity: This antibody is specific for Erythropoetin (EPO) and binds to an Epitope within the first 26 amino acids of the N-terminus of Human urinary and Human recombinant EPO.Aff. Const.:6.7 x 10e-9M (native human EPO).

Purification: Affinity Chromatography on Protein G

Endotoxin Level: < 0.1 EU per 1 μg of antibody as determined by the LAL method

Immunogen: A 26 amino acid synthetic peptide analogue of Erythropoietin / EPO

Isotype: IgG2a

Application Details

Application Notes: Western blotting: Use at approx. 1 μg/mL to detect 10 ng/lane of Human EPO under reducing andnon reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate Buffered Saline (PBS), pH 7.2, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: Erythropoietin / EPO

Target Type: Hormone

Background: EPO is a 30 kDa glycoprotein hormone secreted primarily by the kidney that stimulates bone marrow erythropoiesis in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80 % sequence identity at the amino acid level.Synonyms: Epoetin

Gene ID: 2056

NCBI Accession: NP_000790

UniProt: P01588

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity