CD9 antibody

Catalog No. ABIN111919

This Mouse Monoclonal antibody specifically detects CD9 in FACS, WB, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Dog, Rabbit, Cat and Monkey.

Quick Overview for CD9 antibody (ABIN111919)

Target: CD9

Reactivity: Human, Dog, Rabbit, Cat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: K41

Product Details anti-CD9 Antibody

Specificity: Clone K41 detects CD9. Antigen distribution on Isolated cells: CD9 is mainly expressed on platelets, on early B-cells, and monocytes. Further reactivity could be seen with basophiles and eosinophiles. CD9 is used for the characterization of leukaemias and lymphomas. 90 % of non-T acute lymphoblastic leukaemic cells, 50 % of acute myeloid as well as chronic lymphoid leukaemias express the antigen. Tissue sections: glomeruli, sinus histiocytes, germinal centre, brain white matter, nerves, skin, and vascular smooth muscles.

Purification: Affinity chromatography.

Immunogen: Vero cells

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry on: Frozen sections: 2-5 μg/mL, 1/80-1/200). Paraffin sections: 8 μg/mL, 1/50, pretreatment is not necessary. Suggested positive control: human tonsil. Also works in FACS and Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % thimerosal as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD9

Target: CD9

Alternative Name: CD9

Background: CD9 is a member of the tetraspan or transmembrane 4 superfamily. The family members (CD9, CD63, CD37, Cd53, CD81, CD82, CD151) show a high degree of homology in their membrane-spanning domain. CD9 is the only member with N-linked sugar bound to the first extracellular segment. Tetraspans are expressed by many cell types and show a different subcellular distribution. CD9 is constitutively surface-expressed by platelets and pre-B cells and is present in the alpha-granules of platelets. The participation of different tetraspan members in signalling complexes on B and T cells have been described. Furthermore, association of CD9 with beta1 integrins has been found. Anti CD9 monoclonal antibodies induce homotypic pre-B cell aggregation and fibronectin receptor-mediated adherence of these cells to bone marrow fibroblasts. Recent observations suggest a role of CD9 in adhesion.Synonyms: 5H9 antigen, Cell growth-inhibiting gene 2 protein, GIG2, Leukocyte antigen MIC3, MIC3, Motility-related protein, TSPAN29, Tetraspanin-29, p24

Gene ID: 928

NCBI Accession: NP_001760

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization