ITGAL antibody

Catalog No. ABIN111920

Product Details anti-ITGAL Antibody

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: MEM-25 recognizes the CD11a subunit of the αLβ2 integrin heterodimer, also called lymphocyte-function-associated antigen 1 (LFA-1). CD11a is a general marker of leucocytes in immune and other tissues. Does not react with non-hemopoietic tissue and platelets. The antibody blocks binding of CFA-1 to ICAM-1.

Purification: Affinity chromatography.

Immunogen: Mixed leukocyte preparation.

Clone: MEM-25

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections (0.2 μg/mL, 1/2000). Flow Cytometry. Immunoprecipitation. Suggested positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.6 mL distilled water.

Buffer: PBS buffer pH 7.2 with 0.05 % sodium azide as preservative and 10 mg/mL BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a / ITGAL (ITGAL Products)

Background: CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. The complex is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain

Gene ID: 3683

NCBI Accession: NP_002200

UniProt: P20701

Pathways: Activated T Cell Proliferation, Integrin Complex