CD31 antibody

Catalog No. ABIN111924

This anti-CD31 antibody is a Mouse Monoclonal antibody detecting CD31 in FACS, IHC (p) and IHC (fro). Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for CD31 antibody (ABIN111924)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MEM-05

Product Details anti-CD31 Antibody

Specificity: This antibody is specific for CD31.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Immunogen: Human CD31

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 1-2 μg/mL (1/200-1/400). Immunohistochemistry on Paraffin Sections: 8 μg/mL (1/50). Proteinase K pretreatment forantigen retrieval is recommended. Suggested Positive Control: Human placenta. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat antimouse IgG+IgM (H+L) minimal-cross reaction to human) for 1 h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7 min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1 h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 12. Wash in PBS13. Counterstain with Mayer's hemalum

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water to 0.4 mg/mL.

Buffer: Stock solution contains PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!

References for anti-CD31 antibody (ABIN111924)

Soupene, Serikov, Kuypers: "Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells." in: Journal of lipid research, Vol. 49, Issue 5, pp. 1103-12, (2008) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 / PECAM1

Background: CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). The CD31 antigen is found on cells lining and within the vasculature, and plays a role in the adhesive interaction between adjacent endothelial cells as well as between leukocytes and endothelial cells.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule

Gene ID: 5175, 9606

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization