GFAP antibody

Catalog No. ABIN111957

The Mouse Monoclonal anti-GFAP antibody (Clone GF-01) (ABIN111957) specifically detects GFAP in WB, IHC (p) and IHC (fro). The antibody is reactive with Human, Pig and Cat samples.

Quick Overview for GFAP antibody (ABIN111957)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Pig, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: GF-01

Product Details anti-GFAP Antibody

Specificity: Antibody GF-01 is an excellent tool for the differentiation of tumours of glial or astrocytic origin. The antibody reacts with GFAP, a 50 kDa intermediate filament protein, which is expressed by astrocytes and to a lesser degree by ependymal cells, folliculostellate cells of the anterior pituitary and cerebellar radial glia. The antibody does not react with other intermediate filaments. The antibody also reacts with porcine GFAP.

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry on: Frozen sections: 0.2 μg/mL (1/2000)Paraffin sections: 1 μg/mL (1/400). Proteinase K pretreatment for antigen retrieval isrecommended. Suggested positive control: Human cortex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 with 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.09 % sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.Synonyms: Glial Fibrillary Acidic Protein

Gene ID: 2670

NCBI Accession: NP_001229305

UniProt: P14136