Insulin antibody (INS) Primary Antibody
- Insulin (INS)
- Human, Cow, Pig
- This Insulin antibody is un-conjugated
- Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- The antibody IN-05 is specific for Human insulin. It recognizes also porcine and bovine insulin. No cross-reactions were found with other serum proteins. The antibody can be used for the quantification of human insulin by RIA, and also for receptor-insulin interactions. In normal tissues, IN-05 reacts with normal pancreatic islet beta cells. In tumor tissues it stains insulin secreting neoplasms (insulinomas).
- Affinity Chromatography.
- Application Notes
Suitable for Immunohistochemistry (IHC), RIA and Receptor Studies. Recommended Dilutions for IHC: Frozen sections: 0.2 μg/mL (1/1000)Paraffin sections: 0.2 μg/mL (1/1000). Pretreatment not necessary. Suggested positive control: Human pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.
- For Research Use only
- Restore with 0.5 mL distilled water.
- 0.2 mg/mL
- PBS, pH 7.2 with 5 mg/mL BSA as stabilizer and 09 % Sodium Azide as a preservative.
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 4 °C
- Insulin (INS)
- Alternative Name
- Insulin (INS Antibody Abstract)
- ins1, xins, ins1-a, Insulin, IDDM2, ILPR, IRDN, MODY10, AA986540, Ins-2, InsII, Mody, Mody4, proinsulin, zgc:109842, insulin, insulin precursor, insulin II, preproinsulin, ins, PIN, INS, Ins, INS-IGF2, Ins2
- Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS
- Gene ID
- NCBI Accession
- NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation