MHC Class I, H-2 antibody

Catalog No. ABIN111986

The Rat Monoclonal anti-MHC Class I, H-2 antibody is suitable to detect MHC Class I, H-2 in samples from Mouse. It has been validated for FACS and IHC (fro).

Quick Overview for MHC Class I, H-2 antibody (ABIN111986)

Target: MHC Class I, H-2

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ER-HR52

Product Details

Purification: Affinity Chromatography.

Immunogen: Mouse macrophage precursor cells Remarks: MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta2-microglobulin (11.5 kDa). The epitope recognized by ER-HR52 is resistant to 0.05% glutaraldehyde, 1% paraformaldehyde and acetone.

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 1 μg/mL (1/200). Recommended Positive Control: Mouse Spleen. Does not react on routinely processed Paraffin Sections. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with Frozen, ice-cold Acetone-Fixed Sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer’s hemalum.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and following reconstitution at 2-8 °C. DO NOT FREEZE!

Target Details

Target: MHC Class I, H-2