Iba1 antibody

Catalog No. ABIN112009

This Mouse Monoclonal antibody specifically detects Iba1 in IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for Iba1 antibody (ABIN112009)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1022-5

Product Details anti-Iba1 Antibody

Specificity: The antibody reacts with AIF-1.

Cross-Reactivity (Details): Species reactivity (tested):Human and Rat.

Purification: Affinity Chromatography.

Immunogen: Purified Human recombinant AIF-1. Epitope not further characterized.

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on Paraffin Sctions: 10-20 μg/mL (1/20-1/0). Mcrowavepretreatment for antigen retrieval recommended. Suggested positive control: Human spleen. Does not react on routinely processed Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Immunohistochemitry With Unlabeled Monoclonal Antibodies On Paraffin-EmbeddedTissue Sections1) Deparaffinize slides in chloroform for 30min and rehydrate to distilled water2) Antigen retrieval: microwave treatment (700W) for 15min in 300ml 0. 01M citrate bufferpH 6. 0. 3) Incubate slides for 15min in 200ml methanol/4. 8ml 30%H2O24) Rinse slides 2x in distilled water and for 5min in TBS, pH 7. 55) Block with normal swine serum diluted 1: 10 in Tris buffered saline pH 7. 5, containing0. 1% bovine serum albumin (BSA). Cover sections with NSS and incubate for 15min in ahumid chamber at room temperature (RT). 6) Remove serum from sections (do not rinse)7) Incubate with primary antibody diluted in TBS/0. 1%BSA, pH 7. 5 for 60min in a humidchamber at room temperature8) Rinse sections in TBS, pH 7. 5 for 5min, 2x9) Incubate with secondary antibody (rabbit anti mouse IgG+IgM-biotin) 1: 400 diluted inTBS/0. 1%BSA, pH 7. 5 for 30min in a moist chamber, room temperature. Preincubate nowStreptABComplex /HRPO in TBS/0. 1%BSA, pH 7. 5! 10) Rinse sections in TBS, pH 7. 5 for 5min, 2x11) Incubate with StreptABComplex /HRPO for 30min in a humid chamber at roomtemperature12) Rinse sections in TBS, pH 7. 5 for 5min, 2x13) DAB-staining Prepare 3,3' - diaminobenzidintetrahydrochloride (DAB) according themanufacturers instructions (e. g. DAB tablets) and incubate slides for 1-15 min. Check colorreaction to avoid overstaining. 14) Rinse sections in TBS, pH 7. 5 for 5min, 2x15) Counterstain in haematoxylin for 1min and rinse in cold tap water for 10min16) Mount slides with coverslips

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2, containing 5 mg/mL BSA as stabilizer and 0.01 % Kathon as preservative.

Preservative: Kathon CG

Precaution of Use: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: Allograft Inflammatory Factor-1 is a Ca2+-binding peptide produced by activated macrophages and microglial cells. It has been suggested that AIF-1 expression is associated with chronic inflammatory processes. AIF-1, like the Ca2+-binding peptides MRP8 and MRP14, is expressed by activated macrophages and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts. The recently described apoptosis-inducing factor AIF is not related to AIF-1.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

Gene ID: 199

NCBI Accession: NP_001614

UniProt: P55008

Pathways: Smooth Muscle Cell Migration