AIMP1 antibody

Catalog No. ABIN112011

The Mouse Monoclonal anti-AIMP1 antibody (Clone 546-2) (ABIN112011) specifically detects AIMP1 in WB, IHC (p), EIA and IHC (fro). The antibody is reactive with Human and Rat samples.

Quick Overview for AIMP1 antibody (ABIN112011)

Target: AIMP1 (Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This AIMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 546-2

Product Details anti-AIMP1 Antibody

Specificity: This antibody reacts with Endothelial-Monocyte-Activating Polypeptide II (EMAP II).

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat.

Purification: Affinity Chromatography.

Immunogen: Recombinant Human EMAPII. The epitope has not been further characterized.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 10 μg/mL (1/40)Immunohistochemistry on Paraffin Sections: 10 μg/mL (1/40). Microwave pretreatment forantigen retrieval is recommended. Recommended Positive Control: Rat spleen. Has been described to work in ELISA and Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute by adding 0.5 mL distilled water.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for AIMP1

Target: AIMP1 (Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))

Alternative Name: SCYE1 / EMAP2

Background: EMAPII is a proinflammatory cytokine and chemoattractant of macrophages. The 20 kDa mature peptide binds to endothelial cells and leukocytes like macrophages. EMAP II activates endothelial cells, by elevation of cytosolic free calcium concentration, release of von Willebrand factor, induction of tissue factor, and expression of adhesion molecules E-selectin and P-selectin. It further activates resting monocytes by elevating cytosolic free calcium concentration, inducing TNFalpha and tissue factor and stimulating chemotaxis. EMAP II has been shown in a tumor model to sensitize the local tumor vasculature to the action of TNFalpha, rendering resistant tumors sensitive to immunotherapy by TNFalpha.Synonyms: AIMP1, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, EMAP-2, EMAP-II, Endothelial monocyte-activating polypeptide 2, Endothelial monocyte-activating polypeptide II, Multisynthase complex auxiliary component p43, Small inducible cytokine subfamily E member 1

Gene ID: 9255

NCBI Accession: NP_001135887

UniProt: Q12904