KIT Ligand antibody (Extracellular Domain)

Catalog No. ABIN112014

This anti-KIT Ligand antibody is a Mouse Monoclonal antibody detecting KIT Ligand in WB, EIA, IHC (p) and IHC (fro). Suitable for Human and Primate.

Quick Overview for KIT Ligand antibody (Extracellular Domain) (ABIN112014)

Target: KIT Ligand (KITLG)

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This KIT Ligand antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HKL12

Product Details anti-KIT Ligand Antibody

Binding Specificity: Extracellular Domain

Specificity: This Monoclonal antibody hKL12 is specific for Stem Cell Factor (hSCF). hKL12 is useful for the detection of hSCF in tissues, isolated cells and for studying biological effects of Human Stem Cell Factor in vitro. Antigen distribution on Tissue sections: Bone marrow stromal cells, fibroblasts, and fetal liver cells express SCF. Keratinocytes stain positive.

Cross-Reactivity (Details): Species reactivity (expected):Primates.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Recombinant Human stem cell factor. Remarks: Recombinant Human SFC is an 18.4 kDa polypeptide containing 165 amino acid residues, corresponding to the sequence of the soluble form of SCF. The antigen is a 248 residue protein. The full-length transcript of the corresponding gene product includes a 25-amino acid secretory leader, a 189-amino acid extracellular domain, a 23-amino acid transmembrane region, and a 36-amino acid cytoplasmic tail. The epitope has not been further characterized.

Isotype: IgG1

Application Details

Application Notes: ELISA (0.15 μg/mL). Has been described to work in Wetsern blots and Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: KITLG / SCF

Background: SCF is a transmembrane protein expressed as two alternatively spliced isoforms, either 220 or 248 amino acids, the former lacking a peptidase cleavage site. The cleavage site is responsible for the release of the soluble form of the protein from the cell surface. The first 164 or 165 residues of the extracellular domain comprise soluble SCF, which exists in solution as a noncovalently linked homodimer. Alone, SCF is a weakly proliferative stimulus but acts synergistically with numerous cytokines. SCF is important in germ cell developement, and an indispensable facor for mast cell proliferation an differentiation.Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand

Gene ID: 4254

NCBI Accession: NP_000890

UniProt: P21583

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway