C3 antibody

Catalog No. ABIN112153

This Mouse Monoclonal antibody specifically detects C3 in WB, IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for C3 antibody (ABIN112153)

Target: C3 (Complement Component 3 (C3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: H206

Product Details anti-C3 Antibody

Specificity: H206 allows demonstration of C3 deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d and does not inhibit hemolytic function of C3. The epitope was located on the C-termius of the alpha-chain (39.5 kDa fragment of C3c). Disorders Specifically Detected: Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with Positive Control anti C3b-beta, (H11, Cat. No. BM2132) and Negative Control anti-C3a (H13, Cat. No. BM2131). Activated, deposited C3b bears C3b-alpha and C3b-beta, but no longer C3a. Ubiquitous or non-specifically absorbed C3 is still C3a positive with H 13.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A.

Immunogen: Human complement component C3

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunobloting (Western). Immunohistochemistry on Frozen Sections (also after Fixation with Paraformaldehyde): Working Dilution: 1/10 in PBS, pH 7.4. Incubation Time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL distilled water

Buffer: Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3

Background: The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3

Gene ID: 718

NCBI Accession: NP_000055

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling