SYNPO antibody

Catalog No. ABIN112223

The Mouse Monoclonal anti-SYNPO antibody has been validated for WB, IF, IHC (p) and IHC (fro). It is suitable to detect SYNPO in samples from Human, Mouse, Rat, Cow, Gerbil and Guinea Pig. There are 5+ publications available.

Quick Overview for SYNPO antibody (ABIN112223)

Target: SYNPO (Synaptopodin (SYNPO))

Reactivity: Human, Mouse, Rat, Cow, Gerbil, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYNPO antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: G1D4

Product Details anti-SYNPO Antibody

Specificity: The antibody reacts specifically with Synaptopodin. It recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-Actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of Rat brain. Differentiation dependent expression in cultured hippocampal neurons.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Rat, Mouse, Guinea Pig and Gerbil. Negative with Rabbit, Frog and Chicken.

Purification: Affinity chromatography on Protein A

Immunogen: Isolated Rat kidney glomeruli.

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence. Immunohistochemistry on Frozen SectionsImmunohistochemistry on Paraffin Embedded Tissue: 1/5-1/10 for 1 h at RT (Aftermicrowave treatment). Immunoblotting (Western): 0.1-0.5 μg/mL. The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Buffer: Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

References for anti-SYNPO antibody (ABIN112223)

Tan, Rak-Raszewska, Skovorodkin, Vainio: "Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis." in: Cells, Vol. 9, Issue 2, (2021) (PubMed).

Xu, Chen, Dong, Meyuhas, Chen: "Phosphorylation of ribosomal protein S6 mediates compensatory renal hypertrophy." in: Kidney international, Vol. 87, Issue 3, pp. 543-56, (2015) (PubMed).

Yates, Papakrivopoulou, Long, Goggolidou, Connolly, Woolf, Dean: "The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation." in: Human molecular genetics, Vol. 19, Issue 23, pp. 4663-76, (2010) (PubMed).

Câmpean, Karpe, Haas, Atalla, Peters, Rupprecht, Liebner, Acker, Plate, Amann: "Angiopoietin 1 and 2 gene and protein expression is differentially regulated in acute anti-Thy1.1 glomerulonephritis." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1174-84, (2008) (PubMed).

Lechler, Wu, Bernhardt, Campean, Gastiger, Hackenbeck, Klanke, Weidemann, Warnecke, Amann, Engehausen, Willam, Eckardt, Rödel, Wiesener: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, (2007) (PubMed).

Target Details for SYNPO

Target: SYNPO (Synaptopodin (SYNPO))

Alternative Name: Synaptopodin / SYNPO

Background: Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data), in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).Synonyms: KIAA1029

Gene ID: 11346, 9606

UniProt: Q8N3V7