KRT4 antibody

Catalog No. ABIN112320

This anti-KRT4 antibody is a Mouse Monoclonal antibody detecting KRT4 in WB, FACS, IF, IHC (p) and IHC (fro). Suitable for Human, Dog, Cat and Zebrafish (Danio rerio). This Primary Antibody has been cited in 1 publication.

Quick Overview for KRT4 antibody (ABIN112320)

Target: KRT4 (Keratin 4 (KRT4))

Reactivity: Human, Dog, Cat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 6B10

Product Details anti-KRT4 Antibody

Specificity: This Monoclonal antibody 6B10 reacts exlcusively with Cytokeratin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands are also positive for 6B10.

Cross-Reactivity (Details): Species reactivity (tested):Human, Canine, Feline, Zebrafish.

Purification: Purified

Immunogen: 6B10 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytokeratin preparation extracted from human esophagus.

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry. Immunohistochemistry on Frozen and Tissues (1/25-1/200) using with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent. Immunoblotting (1/100-1/1000). Flow Cytometry (1/25-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-KRT4 antibody (ABIN112320)

Liu, Li, Tan, Beuerman: "The eyelid margin: a transitional zone for 2 epithelial phenotypes." in: Archives of ophthalmology (Chicago, Ill. : 1960), Vol. 125, Issue 4, pp. 523-32, (2007) (PubMed).

Target Details for KRT4

Target: KRT4 (Keratin 4 (KRT4))

Alternative Name: Cytokeratin 4

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK4, CYK4, Cytokeratin-4, K4, KRT4, Keratin, Keratin-4, type II cytoskeletal 4

Gene ID: 9606

UniProt: P19013