Reticulon 1 antibody

Catalog No. ABIN112383

This anti-Reticulon 1 antibody is a Mouse Monoclonal antibody detecting Reticulon 1 in WB, IHC (fro), IHC (p), FACS and IF. Suitable for Human, Mouse, Rat and Hamster.

Quick Overview for Reticulon 1 antibody (ABIN112383)

Target: Reticulon 1 (RTN1)

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Reticulon 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MON160

Product Details anti-Reticulon 1 Antibody

Specificity: MON-160 exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues. The epitope of MON-160 is located between amino acid residues 174-337 of Reticulon-1A.

Purification: Purified

Immunogen: Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).

Isotype: IgG1

Application Details

Application Notes: MON-160 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen sections and paraffin-embedded tissues and Flow cytometry. Recommended dilutions: 1/50-1/100 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/50-1/100 for Immunoblotting applications.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing/thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized antibody at 2-8 °C. After reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -40 °C for longer.

Target Details for Reticulon 1

Target: Reticulon 1 (RTN1)

Alternative Name: Reticulon 1 / RTN1

Background: Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSPA), Reticulon-1B (NSP-B), and Reticulon-1C (NSPC). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).Synonyms: NSP, Neuroendocrine-specific protein, RTN1, Reticulon-1

Gene ID: 6252

NCBI Accession: NP_066959

UniProt: Q16799