Reticulon 1 antibody

Catalog No. ABIN112386

This anti-Reticulon 1 antibody is a Mouse Monoclonal antibody detecting Reticulon 1 in WB, IHC (fro), IHC (p), FACS and IF. Suitable for Human, Rabbit and Monkey.

Quick Overview for Reticulon 1 antibody (ABIN112386)

Target: Reticulon 1 (RTN1)

Reactivity: Human, Rabbit, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Reticulon 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: RNL-2

Product Details anti-Reticulon 1 Antibody

Specificity: RNL-2 recognizes an epitope located within the region of amino acids 421-589 of the neuroendocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rabbit and Rhesus Monkey.

Purification: Purified

Immunogen: The small cell lung cancer cell line NCI-H82.

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunocytochemistry, Immunohistochemistry on frozen tissues,Immunoblotting and Flow cytometry. Recommended dilutions: 1/25-1/200 for Flow cytometry, and for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted -20 °C only.

Target Details for Reticulon 1

Target: Reticulon 1 (RTN1)

Alternative Name: Reticulon 1 / RTN1

Background: Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSPA), Reticulon-1B (NSP-B), and Reticulon-1C (NSPC). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).Synonyms: NSP, Neuroendocrine-specific protein, RTN1, Reticulon-1

Gene ID: 6252

NCBI Accession: NP_066959

UniProt: Q16799