This Rabbit Polyclonal antibody specifically detects Candida albicans in IF, WB, EIA and IHC (fro). It exhibits reactivity toward Candida sp.. It has been mentioned in 2+ publications
Suitable for use in ELISA, IFA (1/1.200), Immunohistochemistry, Double Diffusion and CIE. Also suitable for conjugation purposes. Use neat in gel-precipitin reactions. Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
4-5 mg/mL (OD280 nm, E0.1% = 1.4)
Buffer
0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative without stabilizing proteins
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Goyer, Loiselet, Bon, LOllivier, Laue, Holland, Bonnin, Dalle: "Intestinal Cell Tight Junctions Limit Invasion of Candida albicans through Active Penetration and Endocytosis in the Early Stages of the Interaction of the Fungus with the Intestinal Barrier." in: PLoS ONE, Vol. 11, Issue 3, pp. e0149159, (2016) (PubMed).
Schild, Heyken, de Groot, Hiller, Mock, de Koster, Horn, Rupp, Hube: "Proteolytic cleavage of covalently linked cell wall proteins by Candida albicans Sap9 and Sap10." in: Eukaryotic cell, Vol. 10, Issue 1, pp. 98-109, (2011) (PubMed).
Target
Candida albicans
Target Type
Species
Background
Candida albicans is the most frequently isolated fungal pathogen of Humans, affecting immunocompromised patients ranging from premature infants to AIDS sufferers. Systemic infections have an attributed mortality of 30-50 % . C. albicans is a diploid organism which has eight sets of homologous chromosomes. It has a genome of approximately 16 Mb (haploid), about 30 % greater than S. cerevisiae (baker's yeast).