The Rabbit Polyclonal anti-Listeria Monocytogenes antibody is suitable to detect Listeria Monocytogenes in samples from Listeria monocytogenes. It has been validated for EIA.
Immunohistochemistry on Frozen Sections: 1/20-1/200. Western blot: 1/20-1/200. ELISA: > 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
1-2 mg/mL (OD280 nm, E0.1% = 1.4)
Buffer
PBS with 0.002 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer.
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
Listeria Monocytogenes
Target Type
Bacteria
Background
The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.