Vaccinia Virus antibody

Catalog No. ABIN112851

This anti-Vaccinia Virus antibody is a Rabbit Polyclonal antibody detecting Vaccinia Virus in EIA and IHC (p). Suitable for Vaccinia Virus (VACV). This Primary Antibody has been cited in 6+ publications.

Quick Overview for Vaccinia Virus antibody (ABIN112851)

Target: Vaccinia Virus (VACV) (Vaccina Virus (VACV))

Reactivity: Vaccinia Virus (VACV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vaccinia Virus antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Vaccinia Virus Antibody

Purification: Protein A Chromatography.

Immunogen: Lister Strain (mixture of virions and infected cell polypeptides)

Application Details

Application Notes: ELISA. Immunohistochemistry (Protein K digestion is recommended with Formalin-Fixed ParaffinEmbedded Sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4-5 mg/mL (OD280 nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2, 0.09 % Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Vaccinia Virus antibody (ABIN112851)

Matsuyama, Yasui, Amakishi, Hayashi, Kuroishi, Ishii, Matsukura, Tani, Furuta, Hirayama: "The IgE-dependent pathway in allergic transfusion reactions: involvement of donor blood allergens other than plasma proteins." in: International journal of hematology, Vol. 102, Issue 1, pp. 93-100, (2016) (PubMed).

Miller, Michel, Vogt, Döllinger, Stern, Piesker, Nitsche: "Identification and characterization of a phage display-derived peptide for orthopoxvirus detection." in: Analytical and bioanalytical chemistry, Vol. 406, Issue 29, pp. 7611-21, (2014) (PubMed).

Bruder, Siewert, Obermeier, Malotka, Scheinert, Kellermann, Ueda, Hohlfeld, Dornmair: "Target specificity of an autoreactive pathogenic human γδ-T cell receptor in myositis." in: The Journal of biological chemistry, Vol. 287, Issue 25, pp. 20986-95, (2012) (PubMed).

Hald, Sprinkel, Ray, Serup, Wright, Madsen: "Generation and characterization of Ptf1a antiserum and localization of Ptf1a in relation to Nkx6.1 and Pdx1 during the earliest stages of mouse pancreas development." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 56, Issue 6, pp. 587-95, (2008) (PubMed).

Akazawa, Date, Morikawa, Murayama, Miyamoto, Kaga, Barth, Baumert, Dubuisson, Wakita: "CD81 expression is important for the permissiveness of Huh7 cell clones for heterogeneous hepatitis C virus infection." in: Journal of virology, Vol. 81, Issue 10, pp. 5036-45, (2007) (PubMed).

Kolak, Westerbacka, Velagapudi, Wågsäter, Yetukuri, Makkonen, Rissanen, Häkkinen, Lindell, Bergholm, Hamsten, Eriksson, Fisher, Oresic, Yki-Järvinen: "Adipose tissue inflammation and increased ceramide content characterize subjects with high liver fat content independent of obesity." in: Diabetes, Vol. 56, Issue 8, pp. 1960-8, (2007) (PubMed).

Target Details for Vaccinia Virus

Target: Vaccinia Virus (VACV) (Vaccina Virus (VACV))

Alternative Name: Vaccinia Virus

Target Type: Virus

Background: Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.