Vaccinia Virus antibody (Peroxidase (POD))

Catalog No. ABIN7478353

The Peroxidase (POD)-conjugated Rabbit Polyclonal anti-Vaccinia Virus antibody (ABIN7478353) specifically detects Vaccinia Virus in IHC, WB and EIA. The antibody is reactive with Vaccinia Virus (VACV) samples.

Quick Overview for Vaccinia Virus antibody (Peroxidase (POD)) (ABIN7478353)

Target: Vaccinia Virus (VACV) (Vaccina Virus (VACV))

Reactivity: Vaccinia Virus (VACV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vaccinia Virus antibody is conjugated to Peroxidase (POD)

Application: Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Vaccinia Virus Antibody (Peroxidase (POD))

Specificity: Purified virions

Cross-Reactivity (Details): Does not crossreact with Parainfluenza (1-3), RSV, Adeno, Influenza A&B or HSV1, This antibody is known to react with the B5R and the A33R proteins of the virus. UNINFECTED CELL REACTIVITY: Does not react with uninfected cells. Reactive with the Lister & MVA strains of Vaccinia and Monkeypox

Purification: This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

Immunogen: New York City Board of Health (NYCBOH) strain

Isotype: IgG

Application Details

Application Notes: TITER : >1:1,000 by indirect ELISA. Functions in IFA, IHC & PRNT, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Target Details for Vaccinia Virus

Target: Vaccinia Virus (VACV) (Vaccina Virus (VACV))

Alternative Name: Vaccina Virus

Target Type: Virus