Topoisomerase I (TOP1)
(Topoisomerase (DNA) I (TOP1))
Reactivity
Human
Host
Human
Clonality
Polyclonal
Conjugate
This Topoisomerase I antibody is un-conjugated
Application
Western Blotting (WB), Immunodiffusion (ID), Enzyme Immunoassay (EIA)
Specificity
Shows only the Scl-70 precipitate band when tested using double immunodiffusion against rabbit thymus extract. Specificity is verified by identity of precipitation with CDC-AF reference serum. The titer obtained will vary with the test system, however, undiluted antibody shows a strong Scl-70 precipitate band. Product shows no reactivity at a dilution of 1:100 when tested against purified SSA, SSB, Sm, Sm/RNp and Jo-1 antigen in an ELISA system designed to detect all IgG subclasses.
Purification
Precipitation of Ig fraction from delipidized and defibrinated human plasma.
Suitable for use in Immunodiffusion and ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Reconstitute with 2 mL deionized water.
Buffer
0.01 M PBS, 0.13 M NaCl containing 0.02 % Sodium azide as preservative.
Preservative
Sodium azide
Precaution of Use
Do Not Add Sodium Azide.
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below.
Target
Topoisomerase I (TOP1)
(Topoisomerase (DNA) I (TOP1))