C3 antibody (C-Term)

Catalog No. ABIN113339

The Chicken Polyclonal anti-C3 antibody has been validated for EIA. It is suitable to detect C3 in samples from Human.

Quick Overview for C3 antibody (C-Term) (ABIN113339)

Target: C3 (Complement Component 3 (C3))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This C3 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-C3 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts with Human C3a and C3adesArg (67-76). It does not cross-react with C3 or C4a.

Purification: Affinity Chromatography

Immunogen: Peptide coupled to carrier protein. The antibody was generated against the carboxy-terminal neoepitope which is not exposed on C3, allowing discrimination between C3 and C3a.

Application Details

Application Notes: ELISA. The titer with native human C3a was 1: 5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month. DO NOT FREEZE!

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3

Background: The complement system is part of the innate immune system representing humoral defence. Activation of the complement cascade via the classical, the alternative or the Lectin pathway liberates several biologically active by-products, among them the anaphylatoxin C3a. Measurement of C3a (and C4a) in plasma or other body fluids can yield important clues about the pathological, and particularly also the inflammatory status of an organism. In serum, C3a activity is under strict control of the carboxypeptidase N. This enzyme rapidly cleaves the C-terminal arginine, thereby generating C3adesArg which shows greatly reduced biological activity as compared to C3a. Thus, the biological activity of C3a is focused mainly on its point of generation, while the degradation product C3adesArg remains relatively stable until it is cleared from the circulation.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3

Gene ID: 718

NCBI Accession: NP_000055

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling