Elastin antibody

Catalog No. ABIN113991

This anti-Elastin antibody is a Rabbit Polyclonal antibody detecting Elastin in IHC (p), IF, RIA, IHC (fro) and EIA. Suitable for Human.

Quick Overview for Elastin antibody (ABIN113991)

Target: Elastin (ELN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Elastin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-Elastin Antibody

Specificity: This antibody is specific for Human elastin. Cross-reactivity in RIA at 1:500 dilution: human collagen type I, II, III, IV, V < 0.1 %, human fibronectin < 0.1 % . Cross reaction with rat < 0.1 %.

Purification: Affinity chromatography

Immunogen: Purified elastin from human skin

Application Details

Application Notes: IF: 1: 80. IHC: 1: 500 for paraffin sections, 60 min at RT or 2-8C over night. ELISA: > 1: 200. RIA. Positive control: Human skin or aorta. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute with 0.5 mL distilled water. For further dilution use appropriate antibody diluent.

Concentration: App. 1 mg/mL

Buffer: Phosphate buffered solution, no BSA and preservative added

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Elastin

Target: Elastin (ELN)

Alternative Name: Elastin

Background: Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties.Synonyms: ELN, Tropoelastin

Gene ID: 2006

NCBI Accession: NP_000492

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization