APOA1 antibody (HRP)

Catalog No. ABIN114061

This Sheep Polyclonal antibody specifically detects APOA1 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for APOA1 antibody (HRP) (ABIN114061)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This APOA1 antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-APOA1 Antibody (HRP)

Specificity: This antibody recognizes Apo-A1 and HDL. It does not react with other plasma proteins, including albumin or immunoglobulin fractions. Does not cross react with apolipoprotein B, LDL and VLDL.

Purification: Affinity chromatography

Immunogen: Human Apolipoprotein A1

Application Details

Application Notes: ELISA (1/5,000 - 1/20,000). Can be used in a two site assay with BM785 bound to themicrotitre plate and ABIN114061 for detection. Higher titers can be used when TMB is usedas the substrate). Western blot (1/100 -1/600).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (OD280)

Buffer: PBS containing 50 % glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: Apolipoprotein A I (APO AI)

Background: Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.Synonyms: APOA1, Apo-AI, ApoA-I, ApoAI

Gene ID: 335

NCBI Accession: NP_000030

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism