CD90 antibody

Catalog No. ABIN114254

The Mouse Monoclonal anti-CD90 antibody has been validated for FACS, IHC (fro) and CyTox. It is suitable to detect CD90 in samples from Mouse.

Quick Overview for CD90 antibody (ABIN114254)

Target: CD90 (THY1)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD90 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Clone: 5a-8

Product Details anti-CD90 Antibody

Specificity: This antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].

Purification: Purified

Immunogen: CBA/J

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on frozen sections. Cytotoxicity Analysis (see protocol).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells,representing 1 test). 4. To each tube, add 1. 0 ug of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that tubes are protected from light since most fluorochromes are lightsensitive)7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: Mouse Strain: BALB/cCell Concentration : 1x10e6 cells per testAntibody Concentration Used: 1. 0 ug / 10e6 cellsIsotypic Control: APC Mouse IgG2bCell Source-Percentage of cells stained above control: Thymus: 99. 8%Strain Distribution: Tissue: ThymusCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 ug/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATL

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS with 0.02 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90

Background: CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein

Gene ID: 21838

NCBI Accession: NP_033408

UniProt: P01831

Pathways: Cell-Cell Junction Organization