H-2Dd antibody

Catalog No. ABIN114286

This Mouse Monoclonal antibody specifically detects H-2Dd in CyTox. It exhibits reactivity toward Mouse.

Quick Overview for H-2Dd antibody (ABIN114286)

Target: H-2Dd (MHC Class I H-2Dd)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This H-2Dd antibody is un-conjugated

Application: Cytotoxicity Test (CyTox)

Clone: 34-5-8S

Product Details anti-H-2Dd Antibody

Specificity: This mAb is specific for cells expressing the H-2D antigen coded for by the d haplotype. The reaction pattern of this antibody with a panel of inbred and recombinant haplotypes demonstrates that the antibody detects a private determinant (H-2.4) of the H-2Dd antigen. This antibody can be used to quantitate or eliminate cells bearing H-2Dd (H-2.4) antigen from the appropriate strains of mice.

Purification: Protein G affinity chromatography

Immunogen: Recipient: C3H/HeJ Donor: B6XDBA/2 spleen cells Fusion Partner: SP2/0.Ag14

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OFH-2Dd BEARING LYMPHOCYTES: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium1 orequivalent. Remove red cells and dead cells (where necessary) by purification of viable

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, no preservative.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for H-2Dd

Target: H-2Dd (MHC Class I H-2Dd)

Alternative Name: MHC Class I H-2 Dd

Background: Synonyms: D-D alpha chain, H-2 class I histocompatibility antigen, H-2D(D), H2-D1

Gene ID: 100045864

NCBI Accession: XP_003086970

UniProt: P01900