MHC Class II I-Ad antibody (PE)

Catalog No. ABIN114360

The PE-conjugated Mouse Monoclonal anti-MHC Class II I-Ad antibody (Clone 34-5-3S) (ABIN114360) specifically detects MHC Class II I-Ad in FACS. The antibody is reactive with Mouse samples.

Quick Overview for MHC Class II I-Ad antibody (PE) (ABIN114360)

Target: MHC Class II I-Ad

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: PE

Application: Flow Cytometry (FACS)

Clone: 34-5-3S

Product Details

Purification: Protein G affinity chromatography

Immunogen: BDF splenocytes Remarks: R-Phycoerythrin conjugates are produced under license and protected under Stanford University held patents 4,520,110, 4,542,104, 4,859,582, 5,055,556 (U.S.): 76695 (EPC): 548440 (Australia): 1,179,942 (Canada): and 1,594,827 (Japan).

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 2 - 0. 1 μg of ABIN114360 per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes arelight sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 μl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl ofepropidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS with 0.02 % sodium azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Expiry Date: 6 months

Target Details

Target: MHC Class II I-Ad