Neuroglobin antibody

Catalog No. ABIN115511

The Chicken Polyclonal anti-Neuroglobin antibody has been validated for WB, IHC (fro) and EIA. It is suitable to detect Neuroglobin in samples from Human.

Quick Overview for Neuroglobin antibody (ABIN115511)

Target: Neuroglobin (NGB)

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This Neuroglobin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-Neuroglobin Antibody

Sequence: MERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSFSTVG ESLLYMLEKC LGPAFTPTRA AWSQLYGAVV QAMSRGWDGE

Specificity: This antibody reacts to Neuroglobin.

Purification: Immunoaffinity chromatography

Immunogen: Recombinant human neuroglobin. The Human Neuroglobin is created as a recombinant protein produced in E. coli. It is the 17 kDa protein containing 151 amino acid residues of the Human Neuroglobin. The amino acid sequence of the recombinant human neuroglobin is 100% homologous with the amino acid sequence of the human neuroglobin.

Application Details

Application Notes: Western Blot. Immunohistochemistry. ELISA. The titer of this antibody was determined by indirect ELISA: it is >1: 100,000 for antibodyconcentration 1 mg/mL, 25 ng of antigen are coated per well, and is then defined at a pointof maximal decrease of the titration curve.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.05 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Concentration: 1.0 mg/mL

Buffer: 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 months.

Expiry Date: 6 months

Target Details for Neuroglobin

Target: Neuroglobin (NGB)

Alternative Name: Neuroglobin

Background: Neuroglobin, a 151 amino acid reside protein, mainly expressed in vertebrate brain and retina, is a recently identified member of the globin superfamily. Augmenting O2 supply, neuroglobin promotes survival of neurons upon hypoxic injury, potentially limiting brain damage. Moreover, neuroglobin may be a novel oxidative stress-responsive sensor for signal transduction in the brain. Neuroglobin expression is increased by neuronal hypoxia in vitro and focal cerebral ischemia in vivo, and neuronal survival after hypoxia is reduced by inhibiting neuroglobin expression with an antisense oligodeoxynucleotide and enhanced by neuroglobin overexpression.Synonyms: NGB

Gene ID: 58157

NCBI Accession: NP_067080

UniProt: Q9NPG2