Periostin antibody

Catalog No. ABIN115513

This Rabbit Polyclonal antibody specifically detects Periostin in WB, IHC (p), EIA and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Chicken.

Quick Overview for Periostin antibody (ABIN115513)

Target: Periostin (POSTN)

Reactivity: Human, Mouse, Rat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Periostin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Periostin Antibody

Sequence: MGHHHHHHHH HHSSGHIEGR HMRNNHYDKI LAHSRIRGRD QGPNVCALQQ ILGTKKKYFS TCKNWYKKSI CGQKTTVLYE CCPGYMRMEG MKGCPAVLPI DHVYGTLGIV GATTTQRYSD ASKLREEIEG KGSFTYFAPS NEAWDNLDSD IRRGLESNVN VELLNALHSH MINKRMLTKD LKNGMIIPSM YNNLGLFINH YPNGVVTVNC ARIIHGNQIA TNGVVHVIDR VLTQIGTSIQ DFIEAEDDLS SFRAAAITSD ILEALGRDGH FTLFAPTNEA FEKLPRGVLE RFMGDKVASE ALMKYHILNT LQCSESIMGG AVFETLEGNT IEIGCDGDSI TVNGIKMVNK KDIVTNNGVI HLIDQVLIPD SAKQVIELAG KQQTTFTDLV AQLGLASALR PDGEYTLLAP VNNAFSDDTL SMVQRLLKLI LQNHILKVKV GLNELYNGQI LETIGGKQLR VFVYRTAVCI ENSCMEKGSK QGRNGAIHIF REIIKPAEKS LHEKLKQDKR FSTFLSLLEA ADLKELLTQP GDWTLFVPTN DAFKGMTSEE KEILIRDKNA LQNIILYHLT PGVFIGKGFE PGVTNILKTT QGSKIFLKEV NDTLLVNELK SKESDIMTTN GVIHVVDKLL YPADTPVGND QLLEILNKLI KYIQIKFVRG STFKEIPVTV Y Remarks: The immunizati on antigen (75 kDa) is a protein containing 648 amino acids of recombinan t Human OSF-2 and 23 amino acids, N-Terminal HisTag and Xa - cleavage site (highlighte d). The antigen contains amino acid residues 22 to 669 of the Human OSF-2 precursor. The antigen includes all four fasciclin domains of OSF-2.

Specificity: The antibody recognizes OSF-2.

Purification: Immunoaffinity Chromatography on Immoblized Recombinant Human OSF2

Immunogen: Recombinant Human OSF-2 Source of Antigen: E. coli.

Application Details

Application Notes: ELISA. Western blot (0.1-1 μg/mL). Immunohistochemistry on Paraffin (1/100-1/5,000) and Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Add 0.05 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Buffer: 0.05 M Phosphate Buffer, 0.1 M NaCl, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Stable the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Periostin

Target: Periostin (POSTN)

Alternative Name: Periostin

Background: Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.Synonyms: OSF-2, OSF2, Osteoblast-specific factor 2, PN, POSTN

Gene ID: 10631

NCBI Accession: NP_001129406

UniProt: Q15063