PROX1 antibody (C-Term)

Catalog No. ABIN115694

The Rabbit Polyclonal anti-PROX1 antibody has been validated for WB, IF and IHC (p). It is suitable to detect PROX1 in samples from Human, Mouse and Rat.

Quick Overview for PROX1 antibody (C-Term) (ABIN115694)

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PROX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PROX1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects Prox-1 in the nucleus of lymphatic endothelial cells.

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human, Mouse.

Purification: Protein A Chromatography

Immunogen: Highly pure (>95%) recombinant Human, highly conserved C-terminal part of Prox1 (homeo domain and prospero domain) derived from E.coli.

Application Details

Application Notes: Western blot (1 - 5 μg/mL). Immunofluorescence (1: 750 - 1: 1000). Immunohistochemistry: the antibody works well with paraffin sections. A boiling step isrecommended when staining paraffin-embedded tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: 5 mM PBS, pH 7.4 without stabilizer or preservative

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PROX1

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Alternative Name: PROX-1

Background: Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lympathic vessels from blood vessels. Prox1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells.Synonyms: Homeobox prospero-like protein PROX1, PROX1, Prospero homeobox protein 1

Gene ID: 5629

NCBI Accession: NP_002754

UniProt: Q92786

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation