IL-4 antibody

Catalog No. ABIN115763

The Mouse Monoclonal anti-IL-4 antibody has been validated for Func, EIA and IHC (fro). It is suitable to detect IL-4 in samples from Human.

Quick Overview for IL-4 antibody (ABIN115763)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-4 antibody is un-conjugated

Application: Functional Studies (Func), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IL-4 Antibody

Specificity: Reacts with Interleukin 4.

Purification: Protein A chromatography

Immunogen: Highly pure (>98%) recombinant human IL-4

Isotype: IgG1

Application Details

Application Notes: ELISA: A 1: 10,000 dilution of a 1 mg/mL antibody solution will give satisfactory results in anELISA using a rabbit anti-mouse second antibody conjugated to alkaline phosphatase. Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-4 (0.5 ng/mL), a concentration of 3.0-4.5 μg/mL is required. Western blot: Use at a concentration of 0.20-0.40 μg/mL. Used in conjunction withcompatible secondary reagents the detection limit for recombinant hIL-4 is 0.25-0.50 ng/lane, under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: Interleukin-4 / IL4

Background: IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra

Gene ID: 3565

NCBI Accession: NP_000580

UniProt: P05112

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation