IL-15 antibody

Catalog No. ABIN115774

This Mouse Monoclonal antibody specifically detects IL-15 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for IL-15 antibody (ABIN115774)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-15 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-15 Antibody

Specificity: Recognizes Human Interleukin-15 (IL-15).

Purification: Ammonium Sulfate Precipitation and Ion Exchange Chromatography

Immunogen: Recombinant hIL-15

Isotype: IgG1

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 250 pg/mL of recombinant Human IL-15 when used with Biotinconjugated anti-Human IL-15 antibody (cat. PP1035B) as the detection antibody at aconcentration of approximately 2.0-4.0 μg/mL. Western Blot : To detect Human IL-15 by Western Blot analysis this antibody can be used ata concentration of 0.50-1.0 μg/mL (non-reducing) or at a concentration of 2.0-4.0 μg/mL(reducing). Used in conjunction with compatible secondary reagents the detection limit for

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-15

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Alternative Name: Interleukin-15 / IL15

Background: IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97 % and 73 % amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle. IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.Synonyms: IL-15

Gene ID: 3600

NCBI Accession: NP_000576

UniProt: P40933

Pathways: JAK-STAT Signaling, Glycosaminoglycan Metabolic Process