BAFF antibody

Catalog No. ABIN115775

The Mouse Monoclonal anti-BAFF antibody has been validated for WB and EIA. It is suitable to detect BAFF in samples from Human.

Quick Overview for BAFF antibody (ABIN115775)

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAFF antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-BAFF Antibody

Specificity: This antibody recognizes Human BAFF. Other species not tested.

Purification: Affinity Chromatography Protein G

Immunogen: Highly pure (98%) E.coli derived Recombinant Human BAFF (Cat.-No PA180)

Isotype: IgG1

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 5-6 μg/mL of thisantibody will detect at least 0.5 ng/well of recombinant Human BAFF when used inconjunction with Biotinylated antigen affinity purified anti-human BAFF(PP1203B1/PP1203B1) as the detection antibody at a concentration of at least 0.25-0.50 μg/mL. Western Blot: To detect Human BAFF by Western Blot analysis this antibody can be used ata concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human BAFF is 2.0-4.0 ng/lane, undernon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Concentration: 1.0 mg/mL

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at RT for up to 1 month. Following reconstitution store at 2-8 °C for one month or at -20 °C for longer.

Target Details for BAFF

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: CD257 / BAFF

Background: Members of the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL1 (for TNF- and ApoL- related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NFkB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF also signals through a third TNF receptor BAFFR/BR3. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N terminal kinase (JNK) and to induce apoptosis. The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain.Synonyms: B cell-activating factor, B lymphocyte stimulator, BAFF, BLYS, Dendritic cell-derived TNF-like molecule, TALL1, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, Tumor necrosis factor ligand superfamily member 13B, ZTNF4

Gene ID: 10673

NCBI Accession: NP_001139117

UniProt: Q9Y275

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response