CCL16 antibody

Catalog No. ABIN115778

The Mouse Monoclonal anti-CCL16 antibody has been validated for WB, EIA and Func. It is suitable to detect CCL16 in samples from Human.

Quick Overview for CCL16 antibody (ABIN115778)

Target: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL16 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL16 Antibody

Specificity: This antibody recognises Human LEC.

Purification: Affinity Chromatography on Protein A

Immunogen: Highly pure (>98%) recombinant Human LEC

Isotype: IgG1

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman LEC (100 ng/mL), a concentration of 1.0-5.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1-2 μg/mL of thisantibody will detect at least 0.03 ng/well of recombinant human LEC when used withbiotinylated antigen affinity purified anti-human LEC as the detection antibody at aconcentration of at least 1 μg/mL. Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at aconcentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL16

Target: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Alternative Name: NCC4 / CCL16

Background: LEC (liver expressed cytokine) displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10.Synonyms: C-C motif chemokine 16, Chemokine CC-4, Chemokine LEC, IL-10-inducible chemokine, ILINCK, LCC-1, LCC1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, Monotactin-1, NCC-4, SCYA16

Gene ID: 6360

NCBI Accession: NP_004581

UniProt: O15467