CXCL9 antibody

Catalog No. ABIN115781

The Mouse Monoclonal anti-CXCL9 antibody has been validated for WB, EIA and Func. It is suitable to detect CXCL9 in samples from Human.

Quick Overview for CXCL9 antibody (ABIN115781)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CXCL9 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CXCL9 Antibody

Specificity: Detects Human Monokine induced by interferon-gamma (MIG).

Purification: Affinity Chromatography on Protein G

Immunogen: Highly pure (>98%) E.coli derived recombinant Human MIG

Isotype: IgG1

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of thisantibody will detect at least 1000 pg/mL of recombinant Human MIG when used withbiotinylated anti-Human MIG antibody (cat. PP1055B) as the detection antibody at aconcentration of at least 1 μg/mL. Western Blot: To detect Human MIG by Western Blot analysis this antibody can be used ata concentration of 0.50-2.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hMIG is 0.25-0.50 ng/lane, undernon-reducing conditions and 2.0-4.0 ng/lane, under reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIG (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Alternative Name: CXCL9

Background: MIG (monokine induced by interferon-gamma), a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor. MIG selectively chemoattracts Th1 lymphocytes, and also exerts other activities including inhibition of tumor growth, angiogenesis, and inhibition of colony formation of hematopoietic progenitors. Human MIG is active on murine cells.Synonyms: C-X-C motif chemokine 9, CMK, CXCL9, Gamma-interferon-induced monokine, MIG, SCYB9, Small-inducible cytokine B9

Gene ID: 4283

NCBI Accession: NP_002407

UniProt: Q07325