RANKL antibody

Catalog No. ABIN115783

This Mouse Monoclonal antibody specifically detects RANKL in EIA. It exhibits reactivity toward Human.

Quick Overview for RANKL antibody (ABIN115783)

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RANKL antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-RANKL Antibody

Specificity: This antibody reacts with Human sRANKL.

Purification: Protein A chromatography.

Immunogen: Recombinant human sRank Ligand.

Isotype: IgG1

Application Details

Application Notes: ELISA: (In a sandwich ELISA (assuming 100 l/well), a concentration of 1-2g/mL of this antibodywill detect at least 0.2 ng/well of recombinant human sRank Ligand when used withbiotinylated antigen affinity purified anti-human Rank Ligand as the detection antibody ata concentration of at least 0.5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of of > 0.2 mg/mL.

Buffer: PBS without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for RANKL

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: CD254 / RANKL

Background: RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11

Gene ID: 8600

NCBI Accession: NP_003692

UniProt: O14788

Pathways: NF-kappaB Signaling