RANKL antibody (Internal Region)

Catalog No. ABIN6391341

Product Details anti-RANKL Antibody

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This RANKL antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: TNFSF11 / OPGL

Sequence: DLAKRSKLEA QP

Specificity: This antibody is expected to recognise both reported isoforms (NP_003692.1 and NP_143026.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes of MCF7 and NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RANKL

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: TNFSF11 (TNFSF11 Products)

Synonyms: CD254 antibody, ODF antibody, OPGL antibody, OPTB2 antibody, RANKL antibody, TRANCE antibody, hRANKL2 antibody, sOdf antibody, TNFSF11 antibody, Ly109l antibody, OPG antibody, Trance antibody, cd254 antibody, odf antibody, opgl antibody, optb2 antibody, rankl antibody, sodf antibody, tnfsf11 antibody, trance antibody, TNF superfamily member 11 antibody, tumor necrosis factor (ligand) superfamily, member 11 antibody, TNF receptor superfamily member 11b antibody, TNF superfamily member 11 L homeolog antibody, TNFSF11 antibody, Tnfsf11 antibody, TNFRSF11B antibody, tnfsf11.L antibody

Background: TNFSF11, tumor necrosis factor (ligand) superfamily, member 11 , ODF, OPGL, RANKL, TRANCE, hRANKL2, sOdf, TNF-related activation-induced cytokine, osteoclast differentiation factor, osteoprotegerin ligand, receptor activator of nuclear factor kappa B liga

Gene ID: 8600

NCBI Accession: NP_003692, NP_143026

Pathways: NF-kappaB Signaling