CCL24 antibody

Catalog No. ABIN115834

This Mouse Monoclonal antibody specifically detects CCL24 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for CCL24 antibody (ABIN115834)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL24 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-CCL24 Antibody

Specificity: This antibody detects Human Eotaxin-2.

Purification: Antigen Affinity Chromatography

Immunogen: Highly pure (>98%) recombinant Human Eotaxin-2

Isotype: IgG1

Application Details

Application Notes: ELISA: In a Sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 80.0 pg/well of recombinant Human Eotaxin-2 when used withBiotin anti-Human Eotaxin-2 antibody (cat. AP01125BT) as the detection antibody at aconcentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect Human Eotaxin-2 by Western Blot analysis this antibody can beused at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant Human Eotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Precaution of Use: Do Not Add Sodium Azide

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for CCL24

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Alternative Name: Eotaxin-2 / CCL24

Background: Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both Eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.Synonyms: C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, MPIF-2, MPIF2, Myeloid progenitor inhibitory factor 2, SCYA24, Small-inducible cytokine A24

Gene ID: 6369

NCBI Accession: NP_002982

UniProt: O00175

Pathways: Regulation of Actin Filament Polymerization