FGF9 antibody

Catalog No. ABIN115849

This Rabbit Polyclonal antibody specifically detects FGF9 in WB, EIA and Func. It exhibits reactivity toward Mouseand has been mentioned in 1 publication.

Quick Overview for FGF9 antibody (ABIN115849)

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF9 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-FGF9 Antibody

Specificity: Recognizes Fibroblast Growth Factor 9 (FGF-9).

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) E.coli derived recombinant Mouse FGF-9

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofmFGF-9 (1.50 ng/mL), a concentration of 0.025-0.06 μg/mL of this antibody is required. Sandwich ELISA: To detect Mouse FGF-9 by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Murine FGF-9 (PP002B1 orPP002B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Mouse FGF-9. Western Blot: To detect Mouse FGF-9 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mFGF-9 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-FGF9 antibody (ABIN115849)

Puk, Möller, Geerlof, Krowiorz, Ahmad, Wagner, Adamski, de Angelis, Graw: "The pathologic effect of a novel neomorphic fgf9 allele is restricted to decreased vision and retarded lens growth." in: PLoS ONE, Vol. 6, Issue 8, pp. e23678, (2011) (PubMed).

Target Details for FGF9

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Alternative Name: FGF9

Background: FGF9 is a heparin binding growth factor, which is a member of the FGF family of proteins. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 is produced mainly by neurons and may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.Synonyms: Fibroblast growth factor 9, GAF, Glia-activating factor, HBGF9, Heparin-binding growth factor 9

Gene ID: 14180

NCBI Accession: NP_038546

UniProt: P54130