GM-CSF antibody (Biotin)

Catalog No. ABIN115851

This anti-GM-CSF antibody is a Rabbit Polyclonal antibody detecting GM-CSF in WB and EIA. Suitable for Mouse.

Quick Overview for GM-CSF antibody (Biotin) (ABIN115851)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GM-CSF antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GM-CSF Antibody (Biotin)

Specificity: Detects Murine GM-CSF.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mGM-CSF (murine GM-CSF)

Application Details

Application Notes: ELISA: (Direct): To detect mGM-CSF by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mGM-CSF. (Sandwich): To detect mGM-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Murine GM-CSF (PP003P1 or PP003P2) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantmGM-CSF. Western Blot: To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentrationof 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background: The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

Gene ID: 12981

NCBI Accession: NP_034099

UniProt: P01587

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin