IL-7 antibody

Catalog No. ABIN115889

The Rabbit Polyclonal anti-IL-7 antibody has been validated for WB, EIA and Func. It is suitable to detect IL-7 in samples from Mouse.

Quick Overview for IL-7 antibody (ABIN115889)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IL-7 Antibody

Specificity: This antibody recognizes Mouse Interleukin-7 (IL-7). Other species not tested.

Purification: Affinity Chromatography immobilized Murine IL-7 matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Mouse IL-7 (Cat.-No PA014)

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-7 (0.60 ng/mL),a concentration of 0.012 - 0.02 μg/mL of this antibody is required. ELISA: To detect mIL-7 by direct ELISA (using 100 l/well antibody solution) a concentration of atleast 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mIL-7. Western Blot: To detect mIL-7 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: Interleukin-7 / IL7

Background: Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.Synonyms: IL-7

Gene ID: 16196

NCBI Accession: NP_032397

UniProt: P10168

Pathways: JAK-STAT Signaling