Ccl12 antibody

Catalog No. ABIN115901

The Rabbit Polyclonal anti-Ccl12 antibody has been validated for WB, EIA and Func. It is suitable to detect Ccl12 in samples from Mouse.

Quick Overview for Ccl12 antibody (ABIN115901)

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ccl12 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-Ccl12 Antibody

Specificity: This antibody reacts with Macrophage Chemotactic Protein-5 (MCP-5)

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mMCP-5.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mMCP-5 (100.00 ng/mL), a concentration of 2.0-3.0 μg/mL of this antibody is required. ELISA: To detect mMCP-5 by direct ELISA (using 100 l/well antibody solution) a concentration ofat least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mMCP-5. Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mMCP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Ccl12

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Alternative Name: MCP-5 / CCL12

Background: MCP-5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations.Synonyms: C-C motif chemokine 12. Macrophage Chemotactic Protein-5, CCL-12, MCP-1-related chemokine, MCP5, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, Small-inducible cytokine A12

Gene ID: 100504977

NCBI Accession: NP_035461

UniProt: Q62401

Pathways: Cellular Response to Molecule of Bacterial Origin