G-CSF antibody

Catalog No. ABIN115925

The Rabbit Polyclonal anti-G-CSF antibody has been validated for WB, EIA and Func. It is suitable to detect G-CSF in samples from Mouse.

Quick Overview for G-CSF antibody (ABIN115925)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G-CSF antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-G-CSF Antibody

Specificity: This antibody recognises Murine G-CSF.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mG-CSF

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mG-CSF (0.5 ng/mL),a concentration of 0.005-0.010 μg/mL of this antibody is required. ELISA: (Indirect): To detect mG-CSF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mG-CSF. (Sandwich): To detect mG-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine G-CSF (PP022B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mG-CSF. Western Blot: To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: G-CSF

Background: Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.Synonyms: C17orf33, CSF3, Colony stimulating factor 3, GCSF, Granulocyte colony-stimulating factor, Pluripoietin

Gene ID: 12985

NCBI Accession: NP_034101

UniProt: P09920

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization