CNTF antibody

Catalog No. ABIN115937

The Rabbit Polyclonal anti-CNTF antibody has been validated for WB, EIA, IHC (fro) and Func. It is suitable to detect CNTF in samples from Rat.

Quick Overview for CNTF antibody (ABIN115937)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNTF antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Product Details anti-CNTF Antibody

Specificity: This antibody recognises Rat CNTF.

Purification: Affinity Chromatography.

Immunogen: Highly purified recombinant CNTF.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat CNTF (0.15 ng/mL), a concentration of 0.002-0.005 μg/mL of this antibody is required. ELISAIndirect: To detect Rat CNTF by indirect ELISA (using 100 l/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Rat CNTF. Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 l/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Rat CNTF as a detection antibody, allowsthe detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western BlotTo detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CNTF

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Alternative Name: CNTF

Background: CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity.Synonyms: Ciliary Neurotrophic Factor

Gene ID: 25707

NCBI Accession: NP_037298

UniProt: P20294

Pathways: JAK-STAT Signaling