CCL3 antibody

Catalog No. ABIN115969

This Rabbit Polyclonal antibody specifically detects CCL3 in WB, EIA and Func. It exhibits reactivity toward Rat.

Quick Overview for CCL3 antibody (ABIN115969)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL3 Antibody

Specificity: Recognizes Rat MIP-1 alpha. Other species not tested.

Purification: Affinity Chromatography employing an immobilized Rat MIP1-alpha Matrix

Immunogen: Highly pure (>98%) E.coli recombinant Rat MIP-1-alpha (Cat.-No PA043)

Application Details

Application Notes: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of Rat MIP-1 alpha (100 ng/mL), a concentration of 8.0-10.0 μg/mL of this antibody is required. ElisaIndirect: To detect Rat MIP-1 alpha by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Rat MIP-1 alpha. Sandwich: To detect Rat MIP-1alpha by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Rat MIP-1 alpha (PP038B1 orPP038B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Rat MIP-1alpha. Western BlotTo detect Rat MIP-1alpha by Western Blot analysis this antibody can be used at a

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: MIP1 alpha / CCL3

Background: Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.Synonyms: C-C motif chemokine 3, CCL-3, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, LD78 alpha, MIP-1-alpha, MIP1A, Macrophage Inflammatory Protein 1 alpha, SCYA3, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

Gene ID: 25542

NCBI Accession: NP_037157

UniProt: P50229

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy